Beijing Medical Journal 2005 Vol.27 No.3

Wang Xiaojuan, Song Xueqin, Wang Liqin,et al

Abstract Objective To develop a method of organotypic culture of spinal cord slice and to establish ways to different ventral a-motor neuron and dosal intereuron. Methods The slice culture were prepared with lumbar spinal cord from 8-day-old rat. The survival of a-motor neuron was evaluted by immunohistochemical staining with monunohistochemical staining with monoclonal

Antibody SMI-32, a nonphosphorylated neurofilament marker. The interneurouns in dorsal horn were identified by monoclonal anti-carlretinin staining. Lactate dehydrogenase (LDH) levels in culture for more than 2 months with excellent celluar orgnization and stable populations of ventralamotor neurous and dorsal interneurons. The levels of LDH at different culture times have no significant difference. Conclusion Organotypic spinal cord slice cultures may provide an effective method for physiological and pathological changes, and neuroprotection of spinal cord study.

Key words: Spinal cord Orgnotypic culture Nonphosphorlated neurofilament lactate dehydrogenase Calretinin

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